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A talking dog named Spot fulfills his ultimate wish to become "a real boy" when he hooks up with a mad scientist, Dr. Ivan Krank. With the support of his family--his best friend and master, Leonard, and their mother, fourth grade teacher, Mrs. Helperman--Spot becomes a canine "guinea pig" in Krank's evolutionary experiment. However, Dr. Krank's experiments have had far from perfect results--witness mosquito girl and alligator boy--and although Spot's transformation to human is complete, not all calculations are exactly correct. It'll take his best buddy, Leonard, and his quirky pet pals: a cat named Mr. Jolly, and a boisterous bird named Pretty Boy, to help him out of his "tight Spot" and try to right this genetic wrong.